血液一般检验-lectu.ppt
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1、第二章 血液一般检测,(Complete Blood Count),School of Medical Laboratory Science HuXin 胡 昕,(Blood Routine Test),Contents and Learning Objectives,The measurements in a CBCCriteria for collection of proper specimensReference value for human and blood clinical significance-Be able to interpret CBC information sh
2、eets,What does a complete blood count measure?,What does a complete blood count measure?,How to collect a complete blood count sample?,Sample collection-Site-Strict aseptic technique-?,Blood Service Clinical Laboratory the First Affiliated Hospital,EDTA-K2,CBC:不凝固的人血,How to interpret complete blood
3、count results?,Reference value RBC Hb成年男性(4.0 5.5)1012/L 120 160 g/L 成年女性(3.5 5.0)1012/L 110 150 g/L 新生儿(6.0 7.0)1012/L 170 200 g/L,1.RBC&Hb,贫血(anemia),Thalassemia,1)Hematocrit,Hct(packed cell volume,PCV),2.Other Red cell indices,Elevated or lowered Hct is accord with the change of RBC,so the clinic
4、al significance of HCT can refer to the RBC and Hb,*Remember Plasma,在某些病理情况下,Hb和RBC的浓度不一定能正确反映全身红细胞总容量的多少。1 大量失血(主要是血容量的缩小,血浓度变化很 少,从Hb等数值上很难反映出贫血)2 水潴留(血浆容量大,红细胞容量正常,但红细 胞浓度低,表面看有贫血)3 失水(血浆容量小,浓度偏高,有贫血也看 不出),Hb对贫血程度的判断上优于RBC计数。,2)Mean RBC indices,useful in diagnosing the type of anemia,g/L,3)RBC Di
5、stribution Width,RDW,These cells exhibit a large variation in size.The RDW is a numerical indication of this morphologic abnormality.,This value indicates the degree of red cell size variation or how much difference exists between the largest and smallest red cells.,2)TOTAL LEUKOCYTE COUNT(TLC),法国AB
6、X血球计数仪Micros60,Normal values:4 10109/L,Neutrophil(Ne)Lymphocyte(Ly)Eosinophil(Eo)Basophil(Ba)Monocyte(Mo),DIFFERENTIAL LEUKOCYTE COUNT,Clinical Significance,Ne:a增多:如急性感染或炎症、急性溶血、失血、粒 细胞白血病等。b减少:感染性疾病如病毒感染及伤寒、血液系统 疾病如再障、物理化学因素如接受射线、单核巨噬细胞功能亢进等。Ly:a增多:感染性疾病如病毒感染、肿瘤性疾病、及移植排斥反应等。b减少:应用肾上腺皮质激素、免疫缺陷性疾病等。,
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