螺丝命名细则.doc
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1、 项 目: 螺丝命名规则编号: 制定人: 制定日期:版本:页数: 审核人:审核日期:颁发部门: 一,说明 该规则依据美制、国际标准、德制、日制、英制、国标要求制定。二,命名明细4 X 10 PW A H C (+) 螺丝直径螺丝长度螺丝头型B:球面圆柱头; C:圆柱头; F(K):沉头; H:六角头;HW:六角头带垫圈; O:半沉头; P:平元头; R:半元头;PW:平元头带垫圈; T:大扁头; V:蘑菇头;螺丝牙型A:自攻尖尾(日标第1种)疏 AB:自攻尖尾(日标第4种)密;B:自攻平尾(日标第2种)疏;C:自攻平尾(日标第3种)密;P:双丝牙 HL:高低牙 U:菠萝牙纹;T:自攻平尾切脚
2、AT:自攻丝尖尾切脚 M:机械牙;BTT:B型三角牙 CCT:C型三角牙 PTT:P型三角牙 STT:S型三角牙热处理 H:有热处理 N:没热处理表面处理Zn:白锌 C:彩锌 B:蓝锌 F:黑锌 O:氧化黑 Ni:镍Cu:青铜Br:红铜 P:磷化 备注(+):十字槽 (-):一字槽 (T):菊花槽 (H):内六角 (PZ):米字槽 (+-):+-槽 (Y):Y型槽 (H):H型槽 (L):止退花齿 (WIS):单活动弹垫圈 (WIF):单活动平垫圈 (WIT):单活动外齿垫圈 (W2SF):双活动平弹垫圈 ( W=6mm):垫圈外径等于6mm (SUS):不锈钢(Cu):黄铜(Br):红铜(8
3、.8):8.8级螺丝(10.9):10.9级螺丝 (12.9):12.9级螺丝 (R):其它注释三,附图The authors would like to thank Johns Hopkins University for the TC-1 cells. This work was supportedby a National Health Research Institutes intramural grant (IV-103-PP-22) and grants from the NationalScience Council, which were awarded to Y.C. Son
4、g (NSC 99-2321-B-400-004-MY3) and S.J. Liu (NSC103-2321-B-400-008).Author ContributionsY.C.S. and S.J.L. designed the studies. Y.C.S. performed the research and analyzed the data. Y.C.S. andS.J.L. wrote the manuscript.Additional InformationC57BL/6 mice were immunized subcutaneously (s.c.)once with 1
5、 g of peptide mixed with or without 10 g of CpG adjuvant. After one week, splenocytes wereharvested, and the response of IFN- -secreting cells was determined by ELISPOT after 48 h of peptidestimulation. Briefly, 2 105 splenocytes were incubated with 1 g/ml irrelevant peptide or RAH peptidein an anti
6、-IFN- -coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells wereremoved, and a biotinylated anti-IFN- Ab (eBioscience, San Diego, CA, USA) was added to each well.The plates were incubated at 37 C for 2 h. Following the addition of the avidin-HRP reagent (eBioscience,CA,
7、USA), the assay was developed using a 3-amine-9-ethyl carbazole (AEC; Sigma-Aldrich, MO,USA) staining solution. The reaction was stopped after 46 min by placing the plate under tap water.The spots were counted using an ELISPOT reader (Cellular Technology Ltd., Shaker Heights, OH, USA).For RAH-specif
8、ic T cell staining, spleens were harvested seven days after the immunizations, andRAH-specific CD8+ T cells were detected by tetramer staining using a PE-labeled RAH tetramer(Beckman Coulter, CA, USA) and a FITC-labeled anti-CD8 monoclonal antibody (mAb) (eBioscience,CA, USA). The stained RAH-specif
9、ic CD8+ T cells were analyzed by flow cytometry.Supplementary information accompanies this paper at Competing financial interests: The authors declare no competing financial interests.How to cite this article: Song, Y.-C. and Liu, S.-J. A TLR9 agonist enhances the anti-tumor immunityof peptide and l
10、ipopeptide vaccines via different mechanisms. Sci. Rep. 5, 12578; doi: 10.1038/srep12578 (2015).This work is licensed under a Creative Commons Attribution 4.0 International License.The images or other third party material in this article are included in the articles CreativeCommons license, unless i
11、ndicated otherwise in the credit line; if the material is not included underthe Creative Commons license, users will need to obtain permission from the license holder toreproduce the material. To view a copy of this license, visit expression of anti-apoptotic molecules such as the BCL-2 familymember
12、s BCL-XL and CASP8 and FADD-like apoptosis regulator(CFLAR, best known as cFLIP), thereby allowing CTLs to surviveand reach neoplastic of various TLR agonists promotes the immunogenicity of DC/malignant cell fusions through the upregulation of IL-12.14,18In this setting, we used a de from Coriolus v
13、ersicolor (PSK, which operates as a TLR2 agonist)and lyophilized preparations of a low-virulence strain (Su) ofStreptococcus pyogenes (OK-432, which acts as a TLR4 agonist),both of which can be produced as good manufacturing practice(GMP)-grade agents and have been previously used in the clinic asbi
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